Integrating network pharmacology and experimental verification strategies to reveal the active ingredients and molecular mechanism of Tenghuang Jiangu Capsule against osteoporosis

Tenghuang Jiangu Capsule (THJGC) is a Chinese herbal formula used for the treatment of osteoporosis and osteoarthritis in China, but its mechanism treating not clear. The aim this study was to investigate therapeutic effect THJGC on intrinsic through network pharmacology experimental validation. Drugs potential targets were obtained from several reliable databases pharmacology, these integrated analyzed using bioinformatics molecular docking strategies. Quercetin, lignans kaempferol identified as key components, included Akt1, MAPKs, CASP3. Subsequently, UPLC-MS/MS analysis confirmed presence components osteoporosis. In addition, ex vivo models, it that inhibited H2O2-induced ROS generation apoptosis, reduced OVX-induced bone loss mouse model Our data suggest has antioxidant, formation-promoting, resorption-inhibiting, MC3T3-E1 apoptosis-reducing effects, thus anti-osteoporotic properties. conclusion, may be promising pharmacologic adjuvant